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I investigated seed longevity of two A. tridentata subspecies over a 2-year period by retrieving seed bags that were placed at varying depths and sampling soil and litter fractions of the existing seed bank across six locations in the Great Basin. Artemisia tridentata ssp. wyomingensis and A. t. ssp. vaseyana exhibited patterns of a steadily decreasing abundance of viable seeds on the surface and beneath litter, with 100% loss of surface seed viability in 24 months. However, 29-36% (wyomingensis) and 30-40% (vaseyana) of buried seeds remained viable throughout the duration of the experiment. Abundance of naturally occurring seeds varied considerably among locations and between years across the Great Basin for both subspecies. Loss of viable seeds from the existing seed bank between post-dispersal and pre-dispersal the following season was higher in magnitude for wyomingensis soil fractions compared to litter fractions, and higher overall than for vaseyana.